A principal problem with inserting an unmodified mammalian gene into a BAC, and then getting that gene expressed in bacteria, is that
B) bacteria translate polycistronic messages only.
C) bacteria cannot remove eukaryotic introns.
D) bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.
E) bacterial DNA is not found in a membrane-bounded nucleus and is therefore incompatible with mammalian DNA.
Answer: C