In DNA sequencing by the Sanger (dideoxy) method:
A) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesis of complementary strands.
B) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are then separated by electrophoresis.
C) the dideoxynucleotides must be present at high levels to obtain long stretches of DNA sequence.
D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.
E) the template DNA strand is radioactive.
Answer: D
Biology
- Which of the following statements about adaptive radiation is correct?
- Which organisms are not examples of an adaptive radiation?
- How long ago do scientists believe life first began on Earth?
- What process produces Earth's atmospheric oxygen?
- What evidence points to the date of the appearance of the first oxygen-producing organisms?
- Which of the following best describes the archaeans described in the video?
- The red sedimentary rocks of Western Australia resulted from the combination of oxygen and _____.
- Achaeans can tolerate which of the following extreme habitats?
- Animals first appeared during the _____.
- The Cenozoic era began approximately _____ million years ago.
- ____ were the dominant vertebrate life form during the Mesozoic era.
- Most modern animal phyla evolved during the _____ era.
- Bony fish first evolved during the _____ .
- Life arose during the _____.
- Flowering plants first appeared during the _____.
- The Mesozoic era began approximately _____ million years ago.
- The Precambrian time began at least _____ million years ago.
- Seed plants first appeared during the _____.
- The first prokaryotic cells appeared during the _____.
- Reptiles first appeared during the _____ era.
- We are living during the _____era.
- Dinosaurs went extinct during the _____.
- _____ were the dominant vertebrate life form during the Paleozoic era.
- You are given an unknown organism to identify. It is unicellular and heterotrophic. It is motile, using many short extensions of the cytoplasm, each featuring the 9 + 2 filament pattern. It has well-developed organelles and three nuclei, one large and two small. This organism is most likely to be a member of which group?
- Reinforced, threadlike pseudopods that can perform phagocytosis are generally characteristic of which group?