In a signal transduction cascade using G-proteins and cAMP, which of the following is not a signal amplification step?
- Activation of G-proteins by an activated receptor
- Activation of adenylyl cyclase molecules by G-proteins
- Creation of cAMP molecules by adenylyl cyclase
- Phosphorylation of target proteins by protein kinase A
- All of the above are steps in which amplification occurs.
Answer: 2