Which of the following experiments would indicate a role for calcium in transmitter secretion?

Which of the following experiments would indicate a role for calcium in transmitter secretion?

Which of the following experiments would indicate a role for calcium in transmitter secretion?





  1. Observation of presynaptic depolarizing currents after blockade of sodium channels
  2. Voltage clamp experiments showing voltage-gated calcium channels in the presynaptic terminal
  3. Induction of transmitter release by injection of calcium into the presynaptic terminal
  4. Blockade of transmitter release by injection of calcium buffer into the presynaptic terminal
  5. All of the above

Answer: 5