Anfinsen denatured Ribonuclease by adding urea and beta-mercaptoethanol. When he was ready to allow it to fold back up, how would he have removed the denaturants?

Anfinsen denatured Ribonuclease by adding urea and beta-mercaptoethanol. When he was ready to allow it to fold back up, how would he have removed the denaturants?




A. distillation
B. dialysis
C. centrifugation
D. activated charcoal


Answer: B