When a mixture of proteins is put through a gel filtration column, which sort of protein emerges first?

When a mixture of proteins is put through a gel filtration column, which sort of protein emerges first?

When a mixture of proteins is put through a gel filtration column, which sort of protein emerges first? 




A. positively charged
B. negatively charged
C. small diameter
D. large diameter
E. denatured



Answer: D