You want to isolate the protein Avidin from a mixture of proteins found in chicken eggs. Avidin is a protein which binds tightly to Biotin, a biochemical cofactor. What is the best way to start the isolation?

You want to isolate the protein Avidin from a mixture of proteins found in chicken eggs. Avidin is a protein which binds tightly to Biotin, a biochemical cofactor. What is the best way to start the isolation?




A. boil the mixture in 6M HCl
B. mix in Chymotrypsin
C. use Gel Filtration
D. use Affinity Chromatography
E. use SDS page



Answer: D


Biology

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