What is the principal difference between the Ziehl-Neelsen acid-fast stain technique with Kinyoun acid-fast stain technique?
A. the length that the organisms are stained
B. the type of dye that is used
C. Kinyoun uses a weaker acid (sulfuric acid) rather than HCl
D. Kinyoun uses auramine-rhodamine instead of carbolfuschin as a stain
E. whether or not heat is used to fix slides
Answer: E