Which of the following is not true about Kirby-Bauer disk diffusion method?
A. After inoculation of bacteria, the agar surface is dried for about 5 minutes and the appropriate antibiotic test disks are placed on it.
B. The plate is placed at 35C for 16 to 18 hours of incubation.
C. Bacteria used to swab plate should be at least a week old.
D. The diameters of the zones of inhibition are measured to the nearest millimeter.
Answer: C
Microbiology
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