Assume the two E.coli strains shown below are allowed to conjugate. Hfr: pro+, arg+, his+, lys+, met+, ampicillin-sensitive F: pro-, arg-, his-, lys-, met-, ampicillin-resistant What supplements would you add to glucose minimal salts agar to select for a recombinant cell that is lys+, arg+, amp-resistant?

Assume the two E.coli strains shown below are allowed to conjugate.
Hfr: pro+, arg+, his+, lys+, met+, ampicillin-sensitive
F: pro-, arg-, his-, lys-, met-, ampicillin-resistant
What supplements would you add to glucose minimal salts agar to select for a recombinant cell that is lys+, arg+, amp-resistant?








A) ampicillin, lysine, arginine
B) lysine, arginine
C) ampicillin, proline, histidine, methionine
D) proline, histidine, methionine
E) ampicillin, prolein, histidine, lysine

















Answer: C


Microbiology

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